Roccella montagnei (family Roccelleceae) a fruticose lichen growing luxuriantly along the coastal regions of India. As the Roccella genus has been shown to be biologically active, we assessed the complete biological profile of mangrove associated lichen, R. montagnei. Initially, the hydroalcoholic extract of manglicolous lichen, R. montagnei was prepared and re-extracted with n-hexane, chloroform, ethyl acetate, acetone and methanol. The resultant extracts were evaluated for their phytochemical analysis, antimicrobial (using four different bacterial and two fungal strains by cup-plate method), antimycobacterial (by MABA), antioxidant activity (DPPH, Superoxide and ABTS assays), cytotoxicity activity (by SRB assay using five different cancer cell lines i.e., MCF-7, DLD-1, HeLa, FADU and A549) and the toxicity studies on NHME cell lines. All the screened extracts showed differences in composition and biological activities. The chloroform and methanolic extracts showed potent inhibitory activity against S. aureus. The methanolic extract depicted prominent inhibitory profile against M. tuberculosis stain. The IC50 values of acetone extract was found to 90, 90.2 and 92.7 µg/ml on DPPH, ABTS and superoxide free radicals, respectively, while RC depicted 98.5, 99.8 and 106.5 µg/ml, respectively. Moreover, the chloroform extract showed good degree of specificity towards DLD-1 with IC50 of 20.5 µg/ml and acetone extract towards FADU with IC50 of 42.1 µg/ml. Simultaneously, all the tested extracts depicted low degree of specificity towards NHME cell lines. This study reveals that this mangrove associated lichen, R. montagnei might be a good source of antibacterial, anti-mycobacterial, antiradicals and anticancer agents.
Loading....